1. Berend Snel - Google 학술 검색 - Google Scholar
공동 저자 모두 보기 ; Predicting protein function by genomic context: quantitative evaluation and qualitative inferences. M Huynen, B Snel, W Lathe, P Bork.
Utrecht University - 인용 횟수 29,192번 - Computational Biology - Bioinformatics - Protein Networks - Evolution - Orthology
2. Overview: Generation of Gene Knockout Mice - PMC
The technique of gene targeting allows for the introduction of engineered genetic mutations into a mouse at a determined genomic locus.
The technique of gene targeting allows for the introduction of engineered genetic mutations into a mouse at a determined genomic locus. The process of generating mouse models with targeted mutations was developed through both the discovery of ...
3. Glutamine synthetase (GS) knockout (KO) using CRISPR/Cpf1 ... - Nature
28 jun 2023 · In this study, both GS genes identified on chromosome 5 (GS5) and 1 (GS1) of CHO-S and CHO-K1, were deleted using CRISPR/Cpf1.
The glutamine synthetase (GS)-based Chinese hamster ovary (CHO) selection system is an attractive approach to efficiently identify suitable clones in the cell line generation process for biologics manufacture, for which GS-knockout (GS-KO) CHO cell lines are commonly used. Since genome analysis indicated that there are two GS genes in CHO cells, deleting only 1 GS gene could potentially result in the activation of other GS genes, consequently reducing the selection efficiency. Therefore, in this study, both GS genes identified on chromosome 5 (GS5) and 1 (GS1) of CHO-S and CHO-K1, were deleted using CRISPR/Cpf1. Both single and double GS-KO CHO-S and K1 showed robust glutamine-dependent growth. Next, the engineered CHO cells were tested for their efficiency of selection of stable producers of two therapeutic antibodies. Analysis of pool cultures and subclones after a single round of 25 µM methionine sulfoxinime (MSX) selection indicated that for CHO-K1 the double GS5,1-KO was more efficient as in the case of a single GS5-KO the GS1 gene was upregulated. In CHO-S, on the other hand, with an autologously lower level of expression of both variants of GS, a single GS5-KO was more robust and already enabled selection of high producers. In conclusion, CRISPR/Cpf1 can be efficiently used to knock out GS genes from CHO cells. The study also indicates that for the generation of host cell lines for efficient selection, the initial characterisation of expression levels of the targe...
4. Blame. (Rodger X Reader) - Wattpad
Bevat niet: enzyme R13
Read 𝐁𝐥𝐚𝐦𝐞. (Rodger X Reader) from the story ❥𝙈𝙖𝙝𝙖𝙡 𝙠𝙤 𝙠𝙞𝙩𝐚❥ (𝔻𝔸ℕ𝔻𝕐'𝕊 𝕎𝕆ℝ𝕃𝔻 𝕆ℕ𝔼𝕊ℍ𝕆𝕋𝕊) (Requests Closed To Catch Up) by _Probabl...
5. Gene Cloning, Characterization and Transesterification Reactions of Mgl ...
The nucleotide FASTA file from RAST was loaded using EGGNOG-Mapper [13] to obtain the KO numbers of the annotated genes. The list was loaded using KEGG ...
Lipases and carboxylesterases are enzymes of biotechnological interest both for their reactions and their specificity. They have wide-ranging applications in the food, pharmaceuticals, biodiesel synthesis, and bioremediation industries. For that reason, the strain Neobacillus thermocopriae C255 was isolated from ash from Popocatepetl volcano and studied as a new source of lipolytic enzymes. It was identified using 16S ribosomal RNA and flagellar protein FliF sequence homology, yielding 100% identity. From the sequencing of its genome, an enzyme with lipolytic activity, classified as a monoacylglycerol lipase, and named Mgl-C255, was cloned in E. coli BL21, and then expressed, biochemically characterized, and tested via transesterification reactions with alcohols and monosaccharides. Based on its sequence and structure, it was placed within family V, having a catalytic triad of S90-D207-H237. Biochemical characterization showed its highest activity at 40 °C, pH 7.5 to 8.5, with C-2 length substrate preference. No metal ions or inhibitors influenced lipolytic activity, except for PMSF, SDS, Cu−2, and Hg−2. Mgl-C255 retained about 50% of its activity in non-polar solvents and showed synthetic activity in organic solvents, making it a good candidate for studying its catalytic potential and selectivity.
6. CRISPR Guide - Addgene
This guide will provide a basic understanding of CRISPR biology, introduce the various applications of CRISPR, and help you get started using CRISPR in your ...
Addgene's guide to using CRISPR plasmids in your lab, from knockouts to pooled library screening.
7. Study the Significant Difference Between Cofactor And Coenzyme
Apart from enzymes, some other compounds involved in such reactions are cofactors and coenzymes. Cofactors are non-protein chemical compounds which are ...
Coenzymes are organic molecules, whereas cofactors are inorganic molecule. Explore more differences between two types of non-protein compounds @ BYJU’S
8. Exploration capacity versus specific enzymatic activity of ... - Nature
3 feb 2024 · Specific enzymatic activities were generally negatively affected by C (ANPP) and soil P availability. ECM fungi enhance the specific enzyme ...
Ectomycorrhizal (ECM) fungi are functionally important in biogeochemical cycles in tropical ecosystems. Extracellular enzymatic activity of ECM on a ground-area basis is the product of two attributes; exploration capacity (ECM surface-area) and specific enzymatic activity. Here, we elucidated which attribute better explained the ECM enzymatic activity in response to different levels of soil phosphorus (P) and Nitrogen (N) availability in five Bornean tropical rainforests. We determined the surface area of ECM root tips as well as the enzymatic activities per ECM surface area for carbon (C), N and P degrading enzymes in each site. We evaluated the relationship of ECM enzyme activities with the resource availabilities of C (Above-ground net primary production; ANPP), N, and P of ECM by a generalized linear mixed model. The ECM enzymatic activities on a ground-area basis were more significantly determined by specific enzymatic activity than by the exploration capacity. Specific enzymatic activities were generally negatively affected by C (ANPP) and soil P availability. ECM fungi enhance the specific enzyme activity rather than the exploration capacity to maintain the capacity of nutrient acquisition. The less dependence of ECM fungi on the exploration capacity in these forests may be related to the limitation of C supply from host trees. We highlighted the adaptive mechanisms of ECM fungi on nutrient acquisition in tropical ecosystems through the response of enzymatic activity t...
9. A Coenzyme or metal ion that is tightly bound to enzyme protein is called ...
(B) A complete catalytic active enzyme with its bound prosthetic group is called apoenzyme. Select the correct option. A. (A) is false but (B) is true.
Consider the following statements :A Coenzyme or metal ion that is tightly bound to enzyme protein is called prosthetic groupB A complete catalytic active enzyme with its bound prosthetic group is called apoenzyme.Select the correct option.
10. The ribose methylation enzyme FTSJ1 has a conserved role in ...
We thank Dr. Bernard Moss (NIAID/NIH) for providing FTSJ1 KO HeLa cells. We thank the members of the TErBio laboratory for helpful discussions and reading of ...
FTSJ1 is a conserved human 2'-O-methyltransferase (Nm-MTase) that modifies several tRNAs at position 32 and the wobble position 34 in the anticodon lo
11. Prostate-Specific Antigen (PSA) Test - NCI
11 mrt 2022 · Learn what a PSA test is, plus the limitations and potential harms of the test when used to screen for prostate cancer.
Learn what a PSA test is, plus the limitations and potential harms of the test when used to screen for prostate cancer.
12. Untargeted Metabolomics Reveals Dysregulation of Glycine - MDPI
... and relations of ALDH1L1 to cancer and other diseases linked to this enzyme. ... Heterozygous males and females were intercrossed to obtain knockout (KO) and ...
Background: ALDH1L1 plays a crucial role in folate metabolism, regulating the flow of one-carbon groups through the conversion of 10-formyltetrahydrofolate to tetrahydrofolate and CO2 in a NADP+-dependent reaction. The downregulation of ALDH1L1 promotes malignant tumor growth, and silencing of ALDH1L1 is commonly observed in many cancers. In a previous study, Aldh1l1 knockout (KO) mice were found to have an altered liver metabotype, including significant alterations in glycine and serine. Serine and glycine play crucial roles in pathways linked to cancer initiation and progression, including one-carbon metabolism. Objective/Methods: To further investigate the metabolic role of ALDH1L1, an untargeted metabolomic analysis was conducted on the liver and plasma of both KO and wild-type (WT) male and female mice. Since ALDH1L1 affects glycine- and serine-coupled metabolites and metabolic pathways, correlation analyses between liver glycine and serine with other liver or plasma metabolites were performed for both WT and KO mice. Significantly correlated metabolites were input into MetaboAnalyst 5.0 for pathway analysis to uncover metabolic pathways coupled with serine and glycine in the presence or absence of ALDH1L1 expression. Results: This analysis showed substantial alterations in pathways associated with glycine and serine following ALDH1L1 loss, including the amino acid metabolism, antioxidant pathways, fatty acid oxidation, and vitamin B5 metabolism. These results indicate t...
13. 21 CFR Part 101 -- Food Labeling - eCFR
(12) Dried egg yolks, frozen egg yolks, and liquid egg yolks may be declared as “egg yolks”. (13) [Reserved]. (14) Each individual fat ...
The term principal display panel as it applies to food in package form and as used in this part, means the part of a label that is most likely to be displayed, presented, shown, or examined under customary conditions of display for retail sale. The principal display panel shall be large enough to accommodate all the mandatory label information required to be placed thereon by this part with clarity and conspicuousness and without obscuring design, vignettes, or crowding. Where packages bear alternate principal display panels, information required to be placed on the principal display panel shall be duplicated on each principal display panel. For the purpose of obtaining uniform type size in declaring the quantity of contents for all packages of substantially the same size, the term area of the principal display panel means the area of the side or surface that bears the principal display panel, which area shall be:
14. Urinalysis: Reference Range, Interpretation, Collection and Panels
21 jun 2022 · WBCs contain an enzyme known as leukocyte esterase, which is released when WBCs undergo lysis. Normally, too few WBCs are present in the urine ...
Normal values are as follows: Color – Yellow (light/pale to dark/deep amber) Clarity/turbidity – Clear or cloudy pH – 4.5-8 Specific gravity – 1.
